研究テーマ

実績・論文など

【発表論文（代表的なもの）】

1.Kazuki Y, Yakura Y, Abe S, Osaki M, Kajitani N, Kazuki K, Takehara S, Honma K, Suemori H, Yamazaki S, Sakuma T, Toki T, Shimizu R, Nakauchi H, Yamamoto T and Oshimura M, Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies, Sci. Rep. 2014 Aug 27;4:6136. doi: 10.1038/srep06136.

 2.Tsuji S, Kawamura F, Kubiura M, Hayashi A, Ohbayashi T, Kazuki Y, Chesné C, Oshimura M, Tada M. Dual-Color Fluorescence Imaging to Monitor CYP3A4 and CYP3A7 Expression in Human Hepatic Carcinoma HepG2 and HepaRG Cells. PLoS One. 2014 Aug 7;9(8):e104123. doi: 10.1371/journal.pone.0104123. eCollection 2014.

 3.Iida Y, Kazuki Y, Hayashi M, Ueda Y, Hasegawa M, Kouprina N, Larionov V, Oshimura M. Bi-HAC vector system toward gene and cell therapy. ACS Synth Biol. 2014 Feb 21;3(2):83-90. doi: 10.1021/sb400166j. Epub 2014 Jan 9.

 4.Wakai M, Abe S, Kazuki Y, Oshimura M, Ishikawa F. A human artificial chromosome recapitulates the metabolism of native telomeres in Mammalian cells. PLoS One. 2014 Feb 18;9(2):e88530. doi: 10.1371/journal.pone.0088530. eCollection 2014.

 5.Takehara S, Schulz TC, Abe S, Takiguchi M, Kazuki K, Kishigami S, Wakayama T, Tomizuka K, Oshimura M, Kazuki Y. A novel transchromosomic system: stable maintenance of an engineered Mb-sized human genomic fragment translocated to a mouse chromosome terminal region. Transgenic Res. 2014 Feb 2. [Epub ahead of print]

 6.Kazuki K, Takehara S, Uno N, Imaoka N, Abe S, Takiguchi M, Hiramatsu K, Oshimura M, Kazuki Y. (2013 Dec 6) Highly stable maintenance of a mouse artificial chromosome in human cells and mice. Biochem Biophys Res Commun. 442(1-2):44-50. doi: 10.1016/j.bbrc.2013.10.171. Epub 2013 Nov 9.

 7.Miyake K, Yang C, Minakuchi Y, Ohori K, Soutome M, Hirasawa T, Kazuki Y, Adachi N, Suzuki S, Itoh M, Goto YI, Andoh T, Kurosawa H, Oshimura M, Sasaki M, Toyoda A, Kubota T. (2013 Jun) Comparison of Genomic and Epigenomic Expression in Monozygotic Twins Discordant for Rett Syndrome. PLoS One.;8(6):e66729.

 8.Hashimoto M, Kobayashi K, Watanabe M, Kazuki Y, Takehara S, Inaba A, Nitta S, Senda N, Oshimura M, Chiba K. (2013 Aug) Knockout of mouse Cyp3a gene enhances synthesis of cholesterol and bile acid in the liver. J Lipid Res. 54(8):2060-8. doi: 10.1194/jlr.M033464. Epub 2013 May 24.

 9.Takiguchi M, Kazuki Y, Hiramatsu K, Abe S, Iida Y, Takehara S, Nishida T, Ohbayashi T, Wakayama T, Oshimura M. (2012 Nov) A Novel and Stable Mouse Artificial Chromosome Vector. ACS Synth. Biol.  doi.org/10.1021/sb3000723

10.Yakura Y, Ishihara C, Kurosaki H, Kazuki Y, Komatsu N, Okada Y, Doi T, Takeya H, Oshimura M. (2013 Feb) An induced pluripotent stem cell-mediated and integration-free factor VIII expression system. Biochem Biophys Res Commun. 431(2):336-41. doi: 10.1016/j.bbrc.2012.12.096. Epub 2013 Jan 3.

11.Kazuki Y, Kobayashi K, Aueviriyavit S, Oshima T, Kuroiwa Y, Tsukazaki Y, Senda N, Kawakami H, Ohtsuki S, Abe S, Takiguchi M, Hoshiya H, Kajitani N, Takehara S, Kubo K, Terasaki T, Chiba K, Tomizuka K, Oshimura M. (2013 Feb) Trans-chromosomic mice containing a human CYP3A cluster for prediction of xenobiotic metabolism in humans. Hum Mol Genet. 22(3):578-92. doi: 10.1093/hmg/dds468.

12.Watanabe Y, Kazuki Y, Oshimura M, Ikemura T, Maekawa M. (2012 Nov) Replication timing in a single human chromosome 11 transferred into the Chinese hamster ovary (CHO) cell line. Gene. 510(1):1-6. doi: 10.1016/j.gene.2012.08.045.

13.Nakanishi T, Kokubun K, Oda H, Aoki M, Soma A, Taniguchi M, Kazuki Y, Oshimura M, Sato K.  (2012 Sep) Bioluminescence imaging of bone formation using hairless osteocalcin-luciferase transgenic mice. Bone. Volume 51, number 3:369-75

14.Takehashi M, Tada M, Kanatsu-Shinohara M, Morimoto H, Kazuki Y, Oshimura M, Tada T, Shinohara T. (2012 Mar) Hybridization of Testis-Derived Stem Cells with Somatic Cells and Embryonic Stem Cells in Mice. Biol Reprod. 14;86(6):178. doi: 10.1095/biolreprod.112.098988.

15.Kakeda M, Nagata K, Osawa K, Matsuno H, Hiratsuka M, Sano A, Okazaki A, Shitara S, Nishikawa S, Masuya A, Hata T, Wako S, Osaki M, Kazuki Y, Oshimura M, Tomizuka K. (2011 Nov) A new chromosome 14-based human artificial chromosome (HAC) vector system for efficient transgene expression in human primary cells. Biochem Biophys Res Commun. Volume 415; number 3:439-44

16.Yasunaga M, Oumi N, Osaki M, Kazuki Y, Nakanishi T, Oshimura M, Sato K. (2011 Sep) Establishment and characterization of a transgenic mouse model for in vivo imaging of bmp4 expression in the pancreas. PLoS One. Volume 6, number 9 :e24956.

17.Hiratsuka M, Uno N, Ueda K, Kurosaki H, Imaoka N, Kazuki K, Ueno E, Akakura Y, Katoh M, Osaki M, Kazuki Y, Nakagawa M, Yamanaka S, Oshimura M. (2011 Oct) Integration-Free iPS Cells Engineered Using Human Artificial Chromosome Vectors. PLoS One. Volume 6, number 10 :e25961.

18.Tedesco FS, Hoshiya H, D'Antona G, Gerli MF, Messina G, Antonini S, Tonlorenzi R, Benedetti S, Berghella L, Torrente Y, Kazuki Y, Bottinelli R, Oshimura M, Cossu G. (2011 Aug) Stem cell-mediated transfer of a human artificial chromosome ameliorates muscular dystrophy. Sci Transl Med. Volume 3, number 96 :96ra78. DOI: 10.1126/scitranslmed.3002342

19.Kurosaki H, Hiratsuka M, Imaoka N, Iida Y, Uno N, Kazuki Y, Ishihara C, Yakura Y, Mimuro J, Sakata Y, Takeya H, Oshimura M. (2011 Oct) Integration-free and stable expression of FVIII using a human artificial chromosome. J Hum Genet. Volume 56, number 10 :727-33. doi: 10.1038/jhg.2011.88.

20.Kazuki Y, Oshimura M. (2011 Sep) Human Artificial Chromosomes for Gene Delivery and the Development of Animal Models. Mol Ther. Volume 19, number 9 :1591-601. doi: 10.1038/mt.2011.136.

21.Wang CC, Kazuki Y, Oshimura M, Ikeo K, Gojobori T.　(2011 Aug) Gene dosage imbalance of human chromosome 21 in mouse embryonic stem cells differentiating to neurons. Gene. Volume 481, number 2 :93-101.

22.Yamaguchi S, Niwa R, Kazuki Y, Ohbayashi T.　Application of a bacterial artificial chromosome modification system for a human artificial chromosome vector. Yonago Acta Medica., 2011; 54:21-31

23.Yamaguchi S, Kazuki Y, Nakayama Y, Nanba E, Oshimura M, Ohbayashi T. (2011 Feb ) A method for producing transgenic cells using a multi-integrase system on a human artificial chromosome vector. PLoS One. Volume 6, number 2:e17267.

24.Kazuki Y, Hoshiya H, Takiguchi M, Abe S, Iida Y, Osaki M, Katoh M, Hiratsuka M, Shirayoshi Y, Hiramatsu K, Ueno E,  Kajitani N,Yoshino T, Kazuki K, Ishihara C, Takehara S, Tsuji S, Ejima F, Toyoda A, Sakaki Y, Larionov V, Kouprina N and Oshimura M (2010 Nov) Refined human artificial chromosome vectors for gene therapy and animal transgenesis. Gene therapy. Volume 18, number 4: 384-93. doi:  10.1038/gt.2010.147

25.Iida Y, Kim JH, Kazuki Y, Hoshiya H, Takiguchi M, Hayashi M, Erliandri I, Lee HS, Samoshkin A, Masumoto H, Earnshaw WC, Kouprina N, Larionov V, Oshimura M (2010 Aug) Human Artificial Chromosome with a Conditional Centromere for Gene Delivery and Gene Expression. DNA Res. Volume 17, number 5:293-301.

26.Katoh M, Kazuki Y, Kazuki K, Kajitani N, Takiguchi M, Nakayama Y, Nakamura T, Oshimura M. (2010 May) Exploitation of the interaction of measles virus fusogenic envelope proteins with the surface receptor CD46 on human cells for microcell-mediated chromosome transfer. BMC Biotechnol. Volume 10, number 1:37-43.

27.Takahashi Y., Tsuji S., Kazuki Y., Noguchi M., Arifuku I., Umebayashi Y., Nakanishi T., Oshimura M., Sato K. (2010 Jul.) Development of evaluation system for bioactive substances using human artificial chromosome-mediated osteocalcin gene expression. J Biochem. Volume148, number 1: 29-34.

28.Kazuki Y., Hiratsuka M., Takiguchi M., Osaki M., Kajitani N., Hoshiya H., Hiramatsu K., Yoshino T., Kazuki K., Ishihara C., Takehara S., Higaki K., Nakagawa M., Takahashi K., Yamanaka S. and Oshimura M. (2010 Feb.) Complete genetic correction of iPS cells from Duchenne muscular dystrophy. Mol. Therapy. Volume18, number 2: 386-93. doi:  10.1038/mt.2009.274

29.Kinoshita Y., Kamitani H., Mamun M.H., Wasita B., Kazuki Y., Hiratsuka M., Oshimura M., Watanabe T. (2010 May.)  A gene delivery system with a human artificial chromosome vector based on migration of mesenchymal stem cells towards human glioblastoma HTB14 cells. Neurol Res. Volume 32, number 4: 429-37.

30.Lee J., Kanatsu-Shinohara M., Morimoto H., Kazuki Y., Takashima S., Oshimura M., Toyokuni S., Shinohara T.  (2009 Jul.) Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-cyclin D2 activation. Cell Stem Cell. Volume 2, number 5:76-86.

31.Hoshiya H., Kazuki Y., Abe S., Takiguchi M., Kajitani N., Watanabe Y., Yoshino T., Shirayoshi Y., Higaki K., Messina G., Cossu G., Oshimura M. (2009 Feb.) A highly stable and non-integrated human artificial chromosome (HAC) containing the 2.4Mb entire human dystrophin gene. Mol. Therapy. Volume17, number 2:309-17.

32.Kai Y., Wang CC., Kishigami S., Kazuki Y., Abe S., Takiguchi M., Shirayoshi Y., Inoue T., Ito H., Wakayama T., Oshimura M. (2009 Feb) Enhanced apoptosis during early neuronal differentiation in mouse ES cells with autosomal imbalance. Cell Res. Volume 19, number 2:247-58.

【総説（代表的なもの）】

1. 香月康宏、押村光雄：染色体工学技術とゲノム編集技術の融合による医学・薬学研究への応用---今すぐ始めるゲノム編集、山本卓　編集、実験医学別冊（羊土社）、p81-82 (2014)

2. 香月康宏、押村光雄：遺伝子改変法②HAC/MAC---ES・iPS細胞実験スタンダード、中辻憲夫　監修・末盛博文　編集、実験医学別冊（羊土社）、p300-315（2014）

3. 香月康宏、押村光雄：安全遺伝子導入のためのヒトおよびマウス人工染色体ベクター---In vitro毒性・動態評価の最前線、小島肇夫　監修、シーエムシー出版、p174-182（2013）

4. 宇野愛海、香月康宏、押村光雄：ヒト人工染色体を用いた遺伝子・再生医療へ向けて、再生医療 Vol.12, No.4, p34-51 (2013)

5. 香月康宏、押村光雄：遺伝子導入のための新規ヒト人工染色体ベクター、細胞工学、Vol.31, No.11, p 1260-1267（2012）

6. 押村光雄、香月康宏、宇野愛海：ヒト人工染色体ベクターをもちいたデュシャンヌ型筋ジストロフィー遺伝子治療への挑戦、臨床神経学、Vol.52, No.11, p 1139-1142（2012）

7. 香月康宏、押村光雄：iPS細胞を用いた遺伝子・細胞治療への挑戦、実験医学、Vol.30, No.10, p 1688-1695（2012）

8. 小林カオル、香月康宏：人工染色体ベクターを用いて作製した薬物動体関連遺伝子ヒト化マウス、DMPKニュースレター、Vol.26, No.5, p11-13（2011）

9. 香月康宏、押村光雄：ヒト人工染色体ベクターによる遺伝子細胞治療へ向けて、日本臨床、Vol.69, No.12, p 2142-7（2011）

10. 香月康宏、押村光雄：染色体工学技術による医薬品開発のためのヒト化モデル動物の開発、谷本学校　毒性質問箱、サイエンティスト社、第13号、p124-126（2011）

11. 香月康宏、押村光雄：iPS細胞とヒト人工染色体ベクターを用いた新たな筋ジストロフィー遺伝子治療に向けて、Medical Science Digest、Vol.37, No.10, p5-6（2011）

12. 香月康宏、押村光雄（2011年2月）：ヒト染色体導入ヒト型マウス、---生物機能モデルと新しいリソース・リサーチツール---編集：小幡裕一・城石俊彦・芦川忠夫・田中啓二・米川博道、株式会社エル・アイ・シー、p432-435

13. 山口繁幸、大林徹也、香月康宏、押村光雄：ヒト人工染色体ベクターの利点とその応用、生化学、Vol. 82, No.9, p846-852 (2010)

14. 香月康宏、押村光雄：筋ジストロフィー患者由来のiPS細胞における遺伝子修復〜ヒト人工染色体ベクターによる新たな遺伝子治療戦略の可能性、Medical Bio Vol.7, No.2, p58-63 (2010)

15. 香月康宏、星谷英寿、押村光雄：ヒト人工染色体とiPS細胞を用いた筋ジストロフィー遺伝子治療へ向けて、再生医療 Vol.8, No.3, p21-26 (2009)

16. 香月康宏、押村光雄：幹細胞のゲノム操作：ヒト人工染色体ベクターの開発、ゲノム医学 Vol.9, No.3, p37-40 (2009)